产品货号：
Z25205
中文名称：
RKIP/PEBP1抗体
英文名称：
Anti-RKIP/PEBP1 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-RKIP/PEBP1 Antibody |
| 指标别名 | HCNP;HCNPpp;Neuropolypeptide h3;PBP;PEBP;PEBP 1;PEBP1;Prostatic binding protein;Raf kinase inhibitor protein;RKIP |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse |
| 应用范围 | WB,IHC,IHC-F,ICC,FCM |
| 基因名称 | PEBP1 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | A synthetic peptide corresponding to a sequence of human PBP (DHRGKFKVASFRKKYELRAPVAGTCYQAEWDDYVPKLYEQ). |
| 实际分子量 | 21KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | PEBP1(Phosphatidylethanolamine-binding protein 1),also called PBP,RKIP,inhibits the phosphorylation and activation of MEK by RAF1.PEBP1 is identical to the phosphatidylethanolamine-binding protein (PBP) with a relative molecular mass of 23kD.The PEBP1 gene is mapped on 12q24.23.PEBP1 coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy.PEBP1 overexpression interferes with the activation of MEK and ERK,induction of AP1-dependent reporter genes,and transformation elicited by an oncogenically activated RAF1 kinase.PEBP1 expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines,and maximal RKIP expression correlated perfectly with the onset of apoptosis by Chatterjee et al(2004).RKIP depletion decreased the mitotic index,the number of metaphase cells,traversal times from nuclear envelope breakdown to anaphase,and an override of mitotic checkpoints induced by spindle poisons. |
| Uniprot ID | P30086 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunohistochemistry in frozen section 1:50～400 Immunocytochemistry in fixed cells 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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